Improved method for chick whole-embryo culture using a filter paper carrier
نویسندگان
چکیده
منابع مشابه
The method of rodent whole embryo culture using the rotator-type bottle culture system.
Whole embryo culture (WEC) technique has been developed in 1950's by New and his colleagues, and applied for developmental biology (1). Although development and growth of mammalian embryos are critically dependent on the function of the placenta, WEC technique allows us to culture mouse and rat embryos ex vivo condition during limited periods corresponding to midgestation stages during embryoni...
متن کاملEvaluation and Optimization of Chick Embryo Fibroblasts for Production of a Fowl Pox Vaccine Based on Cell Culture
Abstract Background and Aims: For more than half a century, the production of fowl pox vaccine at Razi Vaccine and Serum Research Institute, has been carried out by injection method in the chick chorioallantoic membrane (CAM) and the vaccine has a favorable and effective in poultry flocks and has provided a complete satisfaction to the poultry flocks owner. Fowl pox vaccine is also manufactured...
متن کاملImproved Assay Technique for Coxiella Burnetii in Chick Embryo.
The egg 50% lethal doses (IiD5o) for Coxiella burnetii range from 105 to 10-6, whereas the 50% infectious doses (ID5o) determined by microscopic examination range from 10-10 to 10-11 (Weiss and Pietryk, J. Bacteriol. 72:235, 1956; Pickens and Gaon, Am. J. Trop. Med. Hyg. 10:49, 1961). If the LDno value for eggs could be increased, dilute suspensions of rickettsiae could be assayed without the t...
متن کاملMethod for electroporation for the early chick embryo.
In vitro whole-embryo culture of chick embryos, originally invented by New, has been widely used for studies of early embryogenesis. Here, a method for electroporation using the New culture and its derivatives is described, to achieve misexpression of exogenous gene in a temporally and spatially controlled manner in gastrulating chick embryos. Detailed information for the devices and procedures...
متن کاملInvestigations into mechanisms of amino acid supply to the rat embryo using whole-embryo culture.
The technique pioneered by D.A.T. New for the in vitro culture of early post-implantation rat embryos has been used to study nutritional mechanisms during early organogenesis. The results indicate that the principal route for amino acid supply to the 8.5- to 11.5-day embryo involves the endocytosis of proteins into cells of the visceral yolk sac endoderm, their digestion in lysosomes, and trans...
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ژورنال
عنوان ژورنال: Developmental Dynamics
سال: 2001
ISSN: 1058-8388,1097-0177
DOI: 10.1002/1097-0177(20010301)220:3<284::aid-dvdy1102>3.0.co;2-5